Fig. 3.

GZY803 mature biofilm showed increased susceptibility to amphotericin B as compared with that of BWP17. A, C. albicans diploid (BWP17) and haploid (GZY803) strains were cultured at 30 °C in GMM (supplemented with required amino acids). Overnight cultures were then used to inoculate and allow biofilm development at 37 °C. Seventy-two-hour biofilms were treated with different concentrations of amphotericin B in RPMI for 24 h and minimum inhibition concentrations were recorded using CFU assays. B, Percentage of surviving biofilm cells at 32 μg/ml was also estimated by comparing the CFU at 32 μg/ml to the fungal count of untreated biofilms. Mean of at least three replicates is shown, with error bars showing S.D. (*): p value <0.05.