Fig. 5.

C. albicans ahp1Δ/Δ biofilm exhibited reduced antioxidant potentials and diminished tolerance and resistance to amphotericin B. The overnight culture of ahp1Δ/Δ (GZY1210) C. albicans strain in GMM (supplemented with amino acids) was used inoculate and allow biofilm development at 37 °C. A, B, Quantification of biofilms formed by BWP17 and ahp1Δ/Δ after 72 h was conducted via XTT and CFU methods. C, Proteins from 72-h biofilms of BWP17 and ahp1Δ/Δ were also extracted and used to estimate the total antioxidant capacity. D, To examine amphotericin B resistance and tolerance, 72-h biofilm samples of BWP17 and ahp1Δ/Δ were treated with different concentrations of amphotericin B in RPMI for 24 h and CFUs were counted by plating the biofilm samples at each concentration on Sabouraud dextrose agar. E, Percentage of surviving biofilm cells at 32 μg/ml was also estimated by comparing the CFUs at 32 μg/ml to the fungal count of untreated biofilms. For all graphs, mean of at least three replicates is shown, with error bars showing S.D. (*): p value <0.05.