Fig. 6.

C. albicans ira2Δ/Δ biofilm showed increased susceptibility to amphotericin B. ira2Δ/Δ (GZY923) C. albicans strain was cultured in GMM supplemented with required amino acids. The overnight culture was used to inoculate and allow biofilm development at 37 °C. Seventy-two-hour biofilms were treated with different concentrations of amphotericin B in RPMI for 24 h and CFUs were recorded by plating the biofilm samples at each concentration on Sabouraud dextrose agar. Parent strain (BWP17) and ira2Δ/Δ rescued strain (ira2Δ/Δ:IRA2, GZY1022) were included as controls. For all graphs, mean of at least three replicates is shown, with error bars showing S.D.