Fig. 7.

IRA2 regulation of AHP1 is linked with C. albicans biofilms' antioxidant potentials and tolerance of amphotericin B. A, ira2Δ/Δ (GZY923) C. albicans strain was cultured in GMM supplemented with required amino acids. The overnight culture was used to inoculate and allow biofilm development at 37 °C. RNA from 72-h biofilm cells was extracted and examined for AHP1 expression using real-time PCR. ACT1 and PMA1 were used as controls. B, To examine amphotericin B resistance and tolerance, 72-h biofilms of BWP17, ira2Δ/Δ and ira2Δ/Δ+TetOff-Myc-AHP1 (GZY1162) were treated with different concentrations of amphotericin B (in the presence or absence of 20 μg/ml of Dox) in RPMI for 24 h and CFUs were recorded by plating the biofilm samples at each concentration on Sabouraud dextrose agar. C, To estimate total antioxidant capacity, proteins from 72-h biofilms of BWP17, ira2Δ/Δ and ira2Δ/Δ+TetOff-Myc-AHP1 (GZY1162) were also used for the antioxidant test. For all graphs, mean of at least three replicates is shown, with error bars showing S.D. (*): p value <0.05.