Table I. Antifungal susceptibility of C. albicans in different modes of growth. Two commonly used C. albicans diploids (SC5314 and BWP17) and two standard haploids (GZY792 and GZY803) were used in this study. Fungal cells cultured overnight at 30 °C in GMM (supplemented with required amino acids) were used to prepare the required inoculum for the antifungal susceptibility test. Minimum inhibition concentration (MIC) was recorded by following the CLSI guideline, or optical density (planktonic), or XTT test (biofilm cells).
MIC (μg/ml) | |||||
---|---|---|---|---|---|
Strains | 30 °C |
37 °C |
Biofilm 107/ml | ||
CLSI 103/ml | Planktonic 107/ml | CLSI 103/ml | Planktonic 107/ml | ||
Amphotericin B | |||||
SC5314 | 0.25 | 0.5 | 0.25 | 0.5 | 16 |
BWP17 | 0.25 | 0.5 | 0.25 | 0.5 | 16 |
GZY792 | 0.25 | 0.5 | 0.25 | 0.5 | 2 |
GZY803 | 0.25 | 0.5 | 0.25 | 0.5 | 2 |
Caspofungin | |||||
SC5314 | 0.5 | 2 | 0.5 | 2 | >32 |
BWP17 | 0.5 | 2 | 0.5 | 2 | >32 |
GZY792 | 0.5 | 2 | 0.5 | 2 | >32 |
GZY803 | 0.5 | 2 | 0.5 | 2 | >32 |
Fluconazole | |||||
SC5314 | 0.5 | 2 | 0.25 | 2 | >32 |
BWP17 | 0.5 | 2 | 0.25 | 2 | >32 |
GZY792 | 0.25 | 2 | 0.25 | 2 | >32 |
GZY803 | 0.25 | 2 | 0.25 | 2 | >32 |
Ketoconazole | |||||
SC5314 | 0.125 | 0.25 | 0.125 | 0.25 | >32 |
BWP17 | 0.125 | 0.25 | 0.125 | 0.25 | >32 |
GZY792 | 0.125 | 0.25 | 0.125 | 0.25 | >32 |
GZY803 | 0.125 | 0.25 | 0.125 | 0.25 | >32 |
Voriconazole | |||||
SC5314 | 0.006 | 0.06 | 0.006 | 0.06 | >32 |
BWP17 | 0.006 | 0.06 | 0.006 | 0.06 | >32 |
GZY792 | 0.006 | 0.06 | 0.006 | 0.06 | >32 |
GZY803 | 0.006 | 0.06 | 0.006 | 0.06 | >32 |