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. 2016 Oct 24;2016:1846296. doi: 10.1155/2016/1846296

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Copyright © 2016 Lu Liu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Experimental protocol diagram. One week after surgery, rats received dura electrical stimulation every 2 days from day 1 to day 5 (days 1, 3, and 5). EA pretreatment was performed every day on both dura stimulation period (before dura electrical stimulation) and blank period (days 0, 2, and 4) in EA group. Spontaneous homecage behaviors recording by HomeCageScan was assessed immediately after dura electrical stimulation (days 1, 3, and 5). Plasma 5-HT immunoreactivity and central 5-HT HPLC determination were performed on day 5.