Figure 4.

Impaired PDE Activity Disrupts the Structural Heterogeneity of Synaptic Boutons

(A) Drosophila wild-type (WT) NMJ on larval muscles 6/7 stained with α-HRP against neuronal membranes (green) and mAb Nc82 against the core active-zone (AZ) protein Bruchpilot (Brp; magenta; Wagh et al., 2006).

(B) Summary bar graphs for the number of Brp puncta (i.e., the number of AZs) per bouton and bouton area.

(C and D) Drosophila dunce¹ NMJ (C) and summary bar graphs (D) as shown in (B).

Scale bars, 5 μm. Differences were statistically significant by the non-parametric Kruskal-Wallis test, followed by Dunn’s multiple comparison test. ^∗∗p < 0.01; ^∗∗∗p < 0.001 versus bouton 1. Data are shown as mean ± SEM (n = 16).