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. 2016 Nov 7;6:36037. doi: 10.1038/srep36037

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Copyright © 2016, The Author(s)

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Generation of TSC1 conditional knockout (CKO) mice in the mesenchymal compartment A: Mouse genotype was detected by PCR. Genomic DNA samples from the indicated strains of mice were analyzed using primers specific for wild-type (WT) and floxed (fl) TSC1, or Cre-ER (T) mice. The PCR products and 100-bp DNA ladder were separated by agarose gel electrophoresis. The bands corresponding to the WT (193 bp) and fl (230 bp) TSC1 alleles are indicated, and confirm the correct TSC1 genotype for each strain. The correct genotype with respect to Cre-ER(T) (500 bp) gene is confirmed in the respective strain. B: TSC1 knockout efficiency was detected by western blotting after daily injection of tamoxifen for 8 days. Liver tissue samples from WT and TSC1 CKO mice were analyzed for p-S6 (s235/236). The phosphorylation status of S6 was elevated, indicating TSC1 knock-down in the livers of TSC1 CKO mice. S6 and GAPDH were included as loading controls.