Table 2. Detection of acrolein-GSH adducts.

Conditions	Proportion of acrolein-GSH adducts detected in solutions [in %]
		Reuterin	3-HPA	Acrolein
pH 4	4 °C	13 ± 4	<11	34 ± 16
pH 4	37 °C	43 ± 19	9 ± 7	72 ± 15
pH 7	37 °C	99 ± 2	92 ± 8	100 ± 0

LC-MRM-MS detection of ions representing acrolein-GSH adducts calculated as proportions of acrolein-GSH adducts relative to all GSH detected in percent. The formation of acrolein-GSH adducts after scavenger addition verified the presence of acrolein or its formation from 3-HPA during incubation (n = 3).

¹0.2 ± 0.1 mM.