Table 3. Antibacterial activity of acrolein-containing solutions.
Strains | MIC50 (μM) |
||||||||
---|---|---|---|---|---|---|---|---|---|
Reuterin |
3-HPA1 |
Acrolein |
|||||||
None | GSH | NAC | None | GSH | NAC | None | GSH | NAC | |
L. innocua | 42.6 ± 26.1 | —2 | —2 | 10.1 ± 0.3 | —2 | —2 | 137.0 ± 46.6 | —2 | —2 |
E. coli | 34.6 ± 8.5 | —2 | —2 | 13.5 ± 1.7 | —2 | —2 | 61.9 ± 27.0 | —2 | —2 |
Minimum inhibitory concentrations (MIC50s) of acrolein present in reuterin or 3-HPA solution, or of pure acrolein toward two indicator strains in the absence (None) and presence of scavenger compounds glutathione (GSH) and N-acetyl cysteine (NAC). Acrolein was quantified in culture medium after reuterin, 3-HPA and acrolein solutions were supplied. Shown are mean MIC50 of at least three independent experiments.
1IC-PAD investigations showed rapid acrolein formation after addition of 3-HPA solutions to LB medium.
2No inhibition of bacterial growth.