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. 2016 Nov 7;6:36551. doi: 10.1038/srep36551

Figure 9. IL-6, IL-8, VEGF and STAT1 expression were augmented in A549-IL-17 cell-bearing nude mouse tissues.

Figure 9

(A,B) The relative protein expression of IL-6, IL-8, VEGF and STAT1 in tumour tissues of A549-IL-17 vs. A549-Neo cell-bearing nude mice was determined by WB. (C) The relative mRNA expression of IL-6, IL-8, VEGF and STAT1 in tumour tissues of A549-IL-17 vs. A549-Neo cell-bearing nude mice was determined by qRT-PCR (n = 5). mRNA expression levels were calculated using the 2−ΔΔCt method, and target gene expression was normalized to the GAPDH housekeeping gene. The data are presented as the mean ± SEM for five mice per group, and the results are representative of two independent experiments. *p < 0.05; **p < 0.01; and ***p < 0.001.