Table 1.
Total AX content per unit dry weight flour in RNAi wheat lines from homozygous (H) and azygous segregant control (A) samples
| Line | mg AX/g flour dwt | ||
|---|---|---|---|
| A | H | H/A % | |
| GT43_2‐3 | 21.0 ± 0.2 | 11.6 ± 0.2 | 55 ± 1% |
| GT43_2‐5 | 23.8 ± 1.5 | 12.4 ± 0.4 | 52%* |
| GT43_2‐6 | 19.8 ± 0.2 | 16.0 ± 0.6 | 81 ± 4% |
| GT47_2‐1 | 21.6 ± 1.0 | 11.6 ± 0.7 | 54 ± 3% |
| GT47_2‐1s | 20.8 ± 0.6 | 13.9 ± 0.5 | 67%* |
| GT47_2‐4 | 19.5 ± 0.1 | 12.4 ± 0.3 | 63 ± 2% |
| GT47_2‐7 | 24.0† | 16.2† | 68% |
| XAT1‐1 | 22.0† | 19.2† | 88% |
| XAT1‐2 | 21.0 ± 1.7‡ | 15.0 ± 0.3‡ | 73 ± 6%‡ |
| XAT1‐3 | 21.0 ± 0.6‡ | 15.1 ± 0.9‡ | 72 ± 3%‡ |
Values (mean ± SE) are from 4 independent biological replicates from randomized block design experiment except where otherwise indicated. Measurements are from pentosan assay for GT43_2 and GT47_2, monosaccharide assay for XAT; these assays give directly comparable results (Saulnier et al., 1995).
*nonblocked design so no replication of H/A estimate. †no biological replication. ‡calculated from previously published data (Anders et al., 2012).