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. 2016 Nov 7;6:36687. doi: 10.1038/srep36687

Figure 1. Human platelets contain miR-30c and PAI-1.

Figure 1

(A) Healthy subject pooled LDP was prepared using CD45+ beads and then characterized using RT-PCR amplification of GPIIb and CD45 mRNAs. (B) Haemocytometer counts verifying successful leukocyte depletion. (C) Validation of sequence-specific human miR-30c and PAI-1 in LDP cDNA libraries by reverse transcription-PCR and agarose gel electrophoresis. I and II are experimental replicates. In the left panel of (C), “M” represents as 20 bp DNA Ladder Marker, “miR-30c” represents as human miR-30c. In the right panel of (C), “M” represents as DL2000 DNA marker. (D) LDP preparation and characterization by RT-PCR amplification of GPIIb and CD45 mRNA and agarose gel electrophoresis analysis in healthy subjects and patients with pre-DM, NCDM and DM-CHD.