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. 2016 Nov 7;6:36687. doi: 10.1038/srep36687

Figure 2. Reciprocal changes of platelet-derived miR-30c and PAI-1 levels in DM2.

Figure 2

(A,B) Reciprocal changes in expression of miR-30c and its target gene PAI-1 mRNA levels were confirmed through qRT-PCR analysis in healthy subjects (control, n = 50), pre-diabetic mellitus subjects (pre-DM, n = 50), non-complicated diabetic subjects (NCDM, n = 40) and diabetes mellitus type 2-coronary heart diabetic subjects (DM-CHD, n = 34) LDPs. The data were normalized to U6 RNA (for miR-30c) and 18S rRNA (for PAI-1) in each sample. (E,F) PAI-1 antigen levels were determined using ELISA following a BCA assay for LDP and PPP in healthy, pre-DM, NCDM and DM-CHD subjects. (G,H) Down- and up-regulation of miR-30c and PAI-1mRNA levels were detected by qRT-PCR in db/db mice LDPs compared to control mice. (I,J) PAI-1 antigen levels were determined by ELISA following a BCA assay in PRP and PPP of db/db mice vs. controls. All data are presented as the mean number per section ±SEM. *p < 0.05 vs. controls. ##p < 0.05 NCDM and DM-CHD subjects vs. pre-DM subjects.