Figure 5. miR-30c modulates thrombus formation in vivo.

(A,B) Carotid arterial occlusion after lenti-miR-30c injection in the FeCl₃-induced injury mouse model. Carotid artery blood flow tracings collected using the Color Laser Doppler Image scanner are shown in the upper panel. The mean times to thrombotic occlusion of carotid arteries obtained from NCD-fed and HFD-fed mice (n = 8) are indicated in the lower panel. *p < 0.05, experimental vs. lenti-NC injection NCD-fed mice and vs. NCD-fed mice. **p < 0.05 vs. lenti-NC injection NC mice and vs. HFD mice. (C,D) The expression of miR-30c and PAI-1 mRNA levels were measured by qRT-PCR in LDPs from HFD-fed and NCD-fed mice after the lenti-miR-30c and lenti-NC injection. (E,F) PAI-1 antigen levels were determined by ELISA following a BCA assay in PRP and PPP. All data are presented as the mean number per section ± SEM. *p < 0.05, experimental vs. lenti-NC injection NCD-fed mice and vs. NCD-fed mice.