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. 2016 Nov 7;6:36576. doi: 10.1038/srep36576

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Copyright © 2016, The Author(s)

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(a,b) Myc-SIRT1 interacts with HA-Nkx2.5 in HEK293A cells. Myc-SIRT1 and HA-Nkx2.5 were transfected individually or co-transfected into HEK293A cells for 48 hours. The cell lysates were immunoprecipitated with anti-Myc (a) or anti-HA (b) antibodies. Then, the immunoprecipitates were subjected to western blot analysis with the indicated antibodies. (c,d) SIRT1 interacts with Nkx2.5 in NRCMs. Primary NRCMs were cultured and lysed for immunoprecipitation with anti-SIRT1 (c) or anti-Nkx2.5 (d) antibodies or control IgG antibody. Then, western blot analysis was performed with the indicated antibodies. (e) Immunofluorescence assay showing SIRT1-Nkx2.5 co-localization in HEK293A cells. Myc-SIRT1 and HA-Nkx2.5 were co-transfected into HEK293A cells for 48 hours, and then immunofluorescence was performed to detect SIRT1 and Nkx2.5. Bar = 10 μm.