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. 2016 Nov 7;6:36576. doi: 10.1038/srep36576

Figure 3. P300 interacts with and acetylates Nkx2.5.

Figure 3

(a) HA-p300 and Myc-Nkx2.5 were co-transfected into HEK293A cells for 48 hours. The cell lysates were immunoprecipitated with anti-Myc or control IgG antibodies. The immunoprecipitates were then subjected to western blot analysis with the indicated antibodies. (b) Myc-Nkx2.5 was transfected into HEK293A cells without or with p300, p300ΔHAT or PCAF for 48 hours. The cell lysates were immunoprecipitated with anti-Myc antibody, followed by western blot with the indicated antibodies. (c) Myc-Nkx2.5 was transfected into HEK293A cells with or without 1 μg (+), 3 μg (++), or 5 μg (+++) of HA-p300 plasmid. The cell lysates were immunoprecipitated with anti-Myc antibody, followed by western blot with the indicated antibodies. (d) Primary neonatal rat cardiomyocytes were infected with adenovirus carrying p300 (Ad-p300) or control GFP (Ad-Ctrl) for 48 hours. The cell lysates were immunoprecipitated with an anti-Nkx2.5 antibody, followed by western blot with the indicated antibodies.