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. 2016 Nov 7;6:36576. doi: 10.1038/srep36576

Figure 6. SIRT1 regulates the transcriptional activity of Nkx2.5.

Figure 6

(a) SIRT1 overexpression represses Nkx2.5-mediated transactivation of the ANF promoter. pGL3-ANF-Luc and pRL-TK were co-transfected into HEK293A cells without or with Nkx2.5 and SIRT1 expression plasmids. Data are expressed as the means ± SEM of three independent experiments. (b) SIRT1 overexpression inhibits the expression of Anf in cardiomyocytes. NRCMs were infected with Ad-SIRT1 or Ad-Ctrl for 48 hours, and then the mRNA expression of Anf was analyzed using a qPCR assay. (c) SIRT1 overexpression represses Nkx2.5-mediated transactivation of the SIRT1 promoter. pGL3-SIRT1-Luc and pRL-TK were co-transfected into HEK293A cells with Nkx2.5 and SIRT1 expression plasmid. The data are expressed as the mean ± SEM of three independent experiments. (d) Sirtinol increases the protein levels of SIRT1 in cardiomyocytes. NRCMs were treated with the indicated concentrations of Sirtinol for 48 hours, and then the protein levels of SIRT1 was analyzed using western blot. (e) Nicotinamide (NAM) increases the protein levels of SIRT1 in cardiomyocytes. NRCMs were treated with the indicated concentrations nicotinamide for 48 hours, and then the protein levels of SIRT1 were determined using western blot.