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. 2016 Nov 7;6:36661. doi: 10.1038/srep36661

Figure 5. C/EBPβ inhibits adipocytes fatty acid oxidation by negative transcriptional expression of Foxc2.

Figure 5

(A) Fragments of Foxc2 promoter fused to a luciferase reporter gene were co-transfected into HEK293T cells together with PGL3-basic (control) or pc-C/EBPβ (n = 3). Luciferase activity was corrected for Renilla luciferase activity and normalized to control activity (n = 3). (B) Chromatin immunoprecipitation (ChIP) analysis of Foxc2 and C/EBPβ (n = 3). (C) Effects of C/EBPβ on FFA level in iWAT adipocytes. Adipocytes were pre-transfected with pc-C/EBPβ, pc-Foxc2 or si-Foxc2 for 72 h (n = 3); (D) Effects of C/EBPβ on palmitate oxidation in iWAT adipocytes. Adipocytes were pre-transfected with pc-C/EBPβ, pc-Foxc2 or si-Foxc2 for 72 h (n = 3); (E) Effects of C/EBPβ on FFA level in iBAT adipocytes. Adipocytes were pre-transfected with pc-C/EBPβ, pc-Foxc2 or si-Foxc2 for 72 h (n = 3); (F) Effects of C/EBPβ on palmitate oxidation in iBAT adipocytes. Adipocytes were pre-transfected with pc-C/EBPβ, pc-Foxc2 or si-Foxc2 for 72 h (n = 3); (G) Effects of C/EBPβ on the protein level of CPT-1, CPT-1 activity and phosphorylation level of ACC in iWAT adipocytes. Adipocytes were pre-transfected with pc-C/EBPβ, pc-Foxc2 or si-Foxc2 for 72 h (n = 3); (H) Effects of C/EBPβ on the protein level of CPT-1, CPT-1 activity and phosphorylation level of ACC in iBAT adipocytes. Adipocytes were pre-transfected with pc-C/EBPβ, pc-Foxc2 or si-Foxc2 for 72 h (n = 3). pc-C/EBPβ: pcDNA3.1-C/EBPβ overexpression vector of C/EBPβ, pc-Foxc2: adenovirus overexpression vector of Foxc2, si-Foxc2: lentiviral interference vector of Foxc2, CPT-1: carnitine palmitoyl transferase-1, ACC: acetyl-CoA carboxylase, the protein expression of CPT-1 was detected by ELISA test. Values are means ± SD. vs. control group, *p < 0.05.