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. 2016 Nov 7;6:36661. doi: 10.1038/srep36661

Figure 6. α-MSH and Foxc2 promote fatty acid oxidation via cAMP/PKA signal pathway in adipocytes.

Figure 6

(A) cAMP level of iWAT adipocytes. Cells were pre-transfected with pc-Foxc2 for 72 h, and then incubated with 500 nM α-MSH for 1 h (n = 3); (B) cAMP level of iBAT adipocytes. Cells were pre-transfected with pc-Foxc2 for 72 h, and then incubated with 500 nM α-MSH for 1 h (n = 3); (C) Protein levels of C/EBPβ, CPT-1 and p-ACC of iWAT adipocytes. Cells were pre-transfected with pc-Foxc2 for 72 h, and then incubated with α-MSH or H89 (n = 3); (D) Protein levels of C/EBPβ, CPT-1 and p-ACC of iBAT adipocytes. Cells were pre-transfected with pc-Foxc2 for 72 h, and then incubated with α-MSH or H89 (n = 3); (E) Protein levels of C/EBPβ, CPT-1 and p-ACC of iWAT adipocytes. Cells were pre-transfected with pc-Foxc2 for 72 h, and then incubated with α-MSH or Forskolin (n = 3); (F) Protein levels of C/EBPβ, CPT-1 and p-ACC of iBAT adipocytes. Cells were pre-transfected with pc-Foxc2 for 72 h, and then incubated with α-MSH or Forskolin (n = 3); (G) Palmitate oxidation in iWAT adipocytes. Cells were pre-transfected with pc-Foxc2 for 72 h, and then incubated with α-MSH or H89 (n = 3); (H) Palmitate oxidation in iBAT adipocytes. Cells were pre-transfected with pc-Foxc2 for 72 h, and then incubated with α-MSH or H89 (n = 3); (I) Palmitate oxidation in iWAT adipocytes. Cells were pre-transfected with pc-Foxc2 for 72 h, and then incubated with α-MSH or Forskolin (n = 3); (J) Palmitate oxidation in iBAT adipocytes. Cells were pre-transfected with pc-Foxc2 for 72 h, and then incubated with α-MSH or Forskolin (n = 3). pc-Foxc2: adenovirus overexpression vector of Foxc2, CPT-1: carnitine palmitoyl transferase-1, ACC: acetyl-CoA carboxylase, Values are means ± SD. vs. control group, *p < 0.05, **p < 0.01.