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. 2016 Nov 7;6:36442. doi: 10.1038/srep36442

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Proteins from pooled H. pylori strains from DU patients or GC patients (n = 5 per group) were digested and labeled separately with different iTRAQ reagents in three independent repeats, then the labeled peptides were combined and separated on a LTQ-Orbitrap Velos hybrid mass spectrometer. Increased expression (>1.5-fold) in GC-derived H. pylori strains than in DU-derived H. pylori strains and proteins related to host signaling by KEGG pathway mapping were used as criteria for selection of candidates, and the candidates were validated by RT-PCR and Western blotting. The selected candidates were then verified by reaction with patients’ serum samples, and a GC-related protein microarray platform was established to perform rapid diagnosis testing.