Figure 2. The intron is efficiently spliced to express GLuc and Core from mcHBV-GLuc cccDNA in HepG2 cells.

(a) GLuc expression in the supernatant of HepG2 cells transfected with mcHBV-GLuc cccDNA at 3 day post transfection. (b) Core expression was detected in HepG2 cells transfected with mcHBV-GLuc cccDNA using western blot (cropped, run in the same gel and in the same experimental conditions). (c) Co-expression of HBc and HBs in transfected cells. HepG2 cells were transfected with mcHBV-GLuc cccDNA for 5 days, fixed and stained with anti-HBc (green) and anti-HBs antibody (red). RLU, relative light units. Data are means ± s.e.m. from triplicate wells and are representative of three independent experiments. Mock: HepG2 cells transfected with pCDNA control. mcHBV-GLuc: HepG2 transfected with mcHBV-GLuc cccDNA.