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. 2016 Nov 7;6:36483. doi: 10.1038/srep36483

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Copyright © 2016, The Author(s)

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(a) HepG2 cells transfected with different plasmids were treated with IFN-beta (500 U/ml) for 4 days at 2 days post transfection. The inhibition of Luciferase gene expression relative to mock treatment was calculated at day 2, 4, 6, 8 and 12 post IFN treatment (D2, D4, D6, D8, and D12). The relative mRNA levels at 12 days post treatment were measured by RT-PCR (b). HBV1.3X:pHBV-1.3X-GLuc, preMC-HBV: pre-mcHBV-GLuc, and mcHBV cccDNA: mcHBV-GLuc cccDNA. CMV-HBV1.05X: pCMV-HBV1.05X-GLuc, CMV-nonHBV: non HBV plasmid pCLucIPZ. **p < 0.01, ***p < 0.001.