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. 2016 Nov 7;6:36483. doi: 10.1038/srep36483

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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1 million HepG2 cells were nucleofected with 2 ug, 0.5 ug, 0.1 ug, 0.02 ug of mcHBV-GLuc cccDNA (H-022 program). Cell concentration was then diluted to 10e5, 10e4, 10e3 and 4 × 10e2 cells/well in 96 well plates. And the GLuc activity was measured at day 3 post transfection. Data was mean ± s.e.m of duplications. BLK: blank control with no transfection.