Fig. 5.

Ca²⁺ uptake by mitochondria. The amount of Ca²⁺ uptake by mitochondria depends on method of CaCl₂ delivery. The bar plots show [Ca²⁺]_m just after addition of CaCl₂ to mitochondria (protocol A, left bar) or after addition of mitochondria to buffer containing CaCl₂ (protocol B, right bar). These data correspond to a time of approximately 65 s. For all 20 and 30 μM CaCl₂ conditions, the rate of Ca²⁺ uptake in protocol A was significantly different from that of protocol B (p ≤ 0.05) for most of the [CaCl₂] and even in the presence of Mg²⁺. Error bars signify standard error of the mean