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. Author manuscript; available in PMC: 2016 Nov 7.

Published in final edited form as: DNA Repair (Amst). 2015 Aug 12;34:39–51. doi: 10.1016/j.dnarep.2015.08.001

R520Q and R295C MUTYH exhibit a significant reduction in substrate affinity. (A) Representative storage phosphor autoradiograms of EMSA with WT, R520Q, and R295C MUTYH and OG:araFA-containing DNA substrate analog duplex at 100 mM NaCl. (B) Representative plot based on EMSA data for K_d determination. Comparison of WT (blue circles), R295C (green triangles), and R520Q (red squares) MUTYH shows that complete binding of the DNA duplex could not be achieved with the R295C or R520Q variants.

R520Q and R295C MUTYH exhibit a significant reduction in substrate affinity. (A) Representative storage phosphor autoradiograms of EMSA with WT, R520Q, and R295C MUTYH and OG:araFA-containing DNA substrate analog duplex at 100 mM NaCl. (B) Representative plot based on EMSA data for K_d determination. Comparison of WT (blue circles), R295C (green triangles), and R520Q (red squares) MUTYH shows that complete binding of the DNA duplex could not be achieved with the R295C or R520Q variants.