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. 2016 Aug 9;8(8):1536–1547. doi: 10.1080/19420862.2016.1219006

Figure 3.

Figure 3.

Cleavage of human IgG1 with variants in the P1 and P2 positions of IdeS. (A) Capillary electrophoresis of antibodies with variants at the P1 and P2 position were digested for 24 hours at 37°C with a 1:10 ratio of IdeS:IgG at 1mg/ml. The P1 and P2 residues are designated in 1-letter code. Leucine and glycine (L235G236) are the natural amino acids at these positions. All antibody variants can be completely cleaved into F(ab′)2 fragments. (B) The cleavage efficiency of the variants is assessed by the amount of F(ab′)2 produced at different IdeS:IgG ratios. While the variant VG is cleaved comparably to the wild-type sequence (LG), other variants require increased amounts of IdeS for complete digestion.