Figure 6.
F(ab′)2 has stronger AHA binding than IgG1 Fab T225 in the AHA ELISA. Titration curves of F(ab′)2 and Fab variants in the AHA ELISA. F(ab′)2has 5-fold higher AHA reactivity than Fab T225. The dilutions corresponding to the OD (1.15) at the middle of the F(ab′)2 titration curves were 70 and 14 for F(ab′)2 and Fab, respectively. F(ab′)2, F(ab′)2 ΔP456, Fab T225, Fab T225L and Fab D221 were coated on the wells. Serial dilutions of pooled human serum were added to the wells and control wells were uncoated. Similar results were obtained in 4 other experiments. The data shown here and in Fig. 1B and Fig. 5D were collected from the same experiment.