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Isoelectric focusing of batches “Newton,” “Darwin” and “Curie” charge variants, positive control (PC) samples and starting material (SM). For batch “Curie” no positive control run was performed due to limited availability of the starting material. More acidic isoforms get enriched in the early eluting fractions, more basic ones are present in higher amounts in the later eluting fractions. No difference is detectable between the starting material and the positive control samples.
