Figure 1.

Effects of additives and pH on the hydrodynamic diameter of mAb-J as measured by dynamic light scattering. (A) Addition of incremental amounts of NaCl and 100 mM arginine to the control buffer and (B) addition of sugars and the effect of pH on the hydrodynamic diameter of mAb-J. All measurements were taken at 25°C. For panel A, mAb-J was prepared at 10 g/L in control solution (20 mM citrate-phosphate buffer containing 30 mM NaCl at pH 6.0) containing either additional NaCl (30, 60 and 100 mM) or 100 mM arginine. For panel B, additional 10% (w/v) sucrose and trehalose were added to the control solution with the pH adjusted to (5.0, 6.0 and 7.0). The error bars represent one standard deviation from 3 independent DLS measurements.