Figure 3.

Effects of additives on the reversible self-association of mAb-J monomers based on analysis of static light scattering showing the mole fractions of monomeric (black squares), dimeric (red circles), and tetrameric (blue triangles) mAb-J species over a protein concentration range of 0.2-20 g/L in (A) control solution, (B) control solution + NaCl, and (C) control solution + arginine. In Panel C, the mole fractions of both dimer and tetramer are superimposed at zero. Samples of mAb-J were prepared in the control solution containing 20 mM citrate-phosphate buffer, 30 mM NaCl, 10% (w/v) trehalose at pH 6.0 with or without additional 100 mM NaCl or arginine. Panel A, B and C show mean of triplicate measurements at 25°C and error bars represent one standard deviation from the calculated mean.