Table 1.
Representative set of APC/C substrates. For a complete list of APC/C substrates and degrons see http://slim.ucd.ie/apc/.
| Activator | Degradation timing | Protein | Regulatory mechanism | Degrons |
|---|---|---|---|---|
| Budding yeast (Saccharomyces cerevisiae) | ||||
| Cdc20 | Pre-metaphase | S-phase cyclin 5 (Clb5) | Degron co-operativity | KEN-like box, D box, ABBA motif, Cks1 co-factor |
| Metaphase, Anaphase | M-phase cyclin 2 (Clb2) | KEN-like box, D box, Cks1 co-factor | ||
| Metaphase | Securin (Pds1) | Phosphorylation | KEN box, D box | |
| Metaphase | Cdc7 kinase regulatory subunit Dbf4 | Phosphorylation | D box | |
| Metaphase | Shugoshin (Sgo1) | D box | ||
| Metaphase | APC/C-Cdh1 modulator 1 (Acm1) | Degron hiding | D box | |
| Cdh1 | Anaphase | APC/C activator protein Cdc20 | KEN-like box, D box | |
| Anaphase | Polo-like protein kinase Cdc5 | KEN box, D boxes | ||
| Anaphase | Filament protein Fin1 | D box | ||
| Anaphase | Kinesin-like protein Kip1 | |||
| Cytokinesis | Contractile ring protein Iqg1 | |||
| Human (Homo sapiens) | ||||
| Cdc20 | Prometaphase | Cyclin A2 (CCNA2) | Degron co-operativity | KEN-like box, D box, ABBA motif, Cks1 co-factor |
| Prometaphase | Protein kinase Nek2 (NEK2) | Degron co-operativity | D box, KEN box, MR tail | |
| Metaphase | Mitotic cyclin B1 (CCNB1) | Degron co-operativity | KEN-like box, D box, Cks1 co-factor | |
| Metaphase | Kinesin-like protein Kif22 (KIF22) | KEN box | ||
| Metaphase | Securin (PTTG1) | Phosphorylation | KEN box, D box | |
| Metaphase | Shugoshin-like 1 (SGOL1) | KEN box, D box | ||
| Cdh1 | Anaphase | Mitotic cyclin B3 (CCNB3) | D box | |
| Anaphase | APC/C activator protein Cdc20 | KEN box | ||
| Anaphase | Kinesin-like protein Kifc1 (KIFC1) | Phosphorylation | D box | |
| Anaphase | Protein kinase Plk1 (PLK1) | D box | ||
| Mitotic exit/G1 | Actin-binding protein anillin (ANLN) | D box | ||
| Mitotic exit/G1 | Aurora kinase B (AURKB) | D box, KEN box | ||
| G1 | Origin-binding protein Cdc6 (CDC6) | Phosphorylation | KEN box, D box | |
| G1 | Geminin (GMNN) | D box | ||
| G1 | S-phase kinase-associated protein 2 (SKP2) | Phosphorylation | D box | |