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. 2016 Nov 3;10:3599–3609. doi: 10.2147/DDDT.S117443

Figure 5.

Figure 5

EGFR shRNA reduced BJ sensitivities by suppressing apoptosis. (A) Viability determination. H1975 cells transfected with EGFR shRNA or NS control for 24 hours were treated with 2 mg/mL of BJ extract for 12 hours and the collected cells counted by trypan blue exclusion assay. Symbol (-) meant no transfection. The results were expressed as mean values from three independent experiments. *P<0.05 and **P<0.01 indicated significant difference between groups. (B) Western blot analysis. Proteins of H1975 cells transfected with EGFR shRNA and NS control before being treated with BJ extract (2 mg/mL) or water were subjected to Western blot analysis. The antibodies included EGFR, phosphorylated EGFRY1068, PARP and loading control GAPDH. Symbol (−) indicated no transfection.

Abbreviations: BJ, Brucea javanica; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PARP, poly(adenosine diphosphate ribose) polymerase; NS, non-specific; shRNA, small hairpin RNA.