Skip to main content
. 2016 Nov 2;10:3545–3553. doi: 10.2147/DDDT.S118768

Figure 2.

Figure 2

Western blot analysis of AKT phosphorylation in HT-22 hippocampal neurons at different times (15, 30, and 60 min) after incubation with GSB-106 (10−8 M), GSB-214 (10−7 M), or BDNF (10−9 M).

Notes: Anti-phospho-AKT1S473, anti-phospho-AKT2S472, and anti-phospho-AKT3S474 were used. Bar graphs represent the intensities of phosphorylated AKT quantified by scanning densitometry of blots, and they are expressed as percentages relative to controls. Total non-phosphorylated GSB-106, hexamethylenediamide and; GSB-214, heptamethylenediamide arbitrary densitometric units were analyzed separately compared to β-actin. Data are presented as the mean ± SD of 5 independent experiments. Differences from the controls were significant at *P<0.05 (Mann–Whitney U-test).

Abbreviations: BDNF, brain-derived neurotrophic factor; GSB-106, bis-(N-monosuccinyl-l-seryl-l-lysine) hexamethylenediamide; GSB-214, bis-(N-monosuccinyl-l-methionyl-l-serine) heptamethylenediamide; SD, standard deviation.