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. 2016 Nov 2;10:3545–3553. doi: 10.2147/DDDT.S118768

Figure 3.

Figure 3

Western blot analysis of ERK phosphorylation in HT-22 hippocampal neurons at different times (15, 30, and 60 min) after incubation with GSB-106 (10−8 M), GSB-214 (10−7 M), or BDNF (10−9 M). Anti-phospho-ERK1/2Y204 antibodies were used. Bar graphs represent intensities of phosphorylated ERK quantified by scanning densitometry of blots, and these intensities are expressed as percentages relative to controls.

Notes: Total non-phosphorylated ERK arbitrary densitometric units were analyzed separately compared to β-actin. The data are presented as the means ± SD of five independent experiments. Differences from the controls were significant at *P<0.05 (Mann–Whitney U-test).

Abbreviations: BDNF, brain-derived neurotrophic factor; ERK, extracellular signal-regulated kinase; GSB-106, bis-(N-monosuccinyl-l-seryl-l-lysine) hexamethylenediamide; GSB-214, bis-(N-monosuccinyl-l-methionyl-l-serine) heptamethylenediamide; SD, standard deviation.