Fig. 3.

Effects of 3-DSC on reporter gene assay. WNT reporter NIH3T3 cells permanently transfected with TCF/LEF-luciferase construct or HEK293 cells stably transfected with STAT3-luciferase constructs were seeded at 2×10⁴ cells in 96-well plate and HEK293 cells permanently transfected with IL-4R site-TKluc/STAT6 contained IL-4 receptor site were seeded at 1×10⁴ cells in each well of a 96-well plate in DMEM containing 5% FBS for 24 h. (A) TCF/LEF reporter gene assay: cells were treated with 3-DSC (0.01–10 μM) for 24 h. (B) IL-6 induced STAT3. (C) IL-4 induced STAT6 reporter gene assay as determined by luciferase activity. Each assay is representative for 3 experiments. The asterisk indicates a significant statistical significance (^*p<0.05).