Fig. 4.

Effects of 3-DSC on hair growth regulation protein expression. HDPCs incubated with various concentrations of 3-DSC (0.1–3 μM) or tofacitinib (0.4 μM) in serum free medium for 48 hr. STAT3 and STAT6 were stimulated by IL-6 (20 ng/ml) or IL-4 (100 ng/ml), respectively, 30 min prior to cell harvest. (A) The level of p-β-catenin and β-catenin was detected by western blotting using specific antibodies in HDPCs. (B) The level of p-STAT3 and STAT3 was detected by western blotting using specific antibodies in IL-6-induced HDPCs. (C) The level of p-STAT6 and STAT6 was detected by western blotting using specific antibodies in IL-4-induced HDPCs. β-Actin protein was used as an internal control. Immunoblot assay was performed as described in the Materials and Methods. Each blot is representative for 3 experiments.