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iSCAT imaging of gold-labeled dynein. 30 nm gold-labeled dynein bound to a microtubule. In this assay, a gold label produces a positive iSCAT contrast when placed into focus (bright) due to the increased distance between the label and the coverglass surface, in contrast to myosin-5a head-labeled gold, which invariably appeared dark (Fig. 2). Scale bars: 1 µm.
