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Western blot analysis of Dex-induced GR phosphorylation in IL-2 plus IL-4 activated eosinophils. Freshly isolated eosinophils were cultured for 16 h in the presence of IL-2 plus IL-4 or control carrier followed by 1 h treatment with Dex at the concentration of 1 µM. Whole cell lysates were analyzed for the phosphorylation of the GCR using phospho-specific antibodies as indicated. These results are representative of three independent experiments.
