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. Author manuscript; available in PMC: 2017 May 15.
Published in final edited form as: J Immunol. 2016 Oct 19;197(10):4042–4052. doi: 10.4049/jimmunol.1601132

Figure 2. Nef selectively colocalizes with Lck traffic regulators Rab11b and MAL.

Figure 2

(A) Deconvoluted confocal images of Jurkat and primary T cells illustrating the degree of Nef co-localization (in white) with: top panel, the Lck traffic regulators Rab11b/MAL in Lck deficient JCAM1.6 and in primary CD4 T cells; and, middle panels, with the LAT traffic regulators Rab27/Rab37 in cells co-expressing or not LATTM-Lck. Bottom panels show the degree of Gts-Nef colocalization with the Lck traffic regulators Rab11b/MAL. Right images show inset amplification.

(B) Population analysis of the volume of co-localization in cells treated as in (A) for n=10 per experimental condition.