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. 2016 Oct 17;113(44):12532–12537. doi: 10.1073/pnas.1610258113

Fig. 3.

Fig. 3.

Immunization with peptide 1 induces a strong anti-VEGF neutralizing antibody response. (A) Immunization of Wistar rats with peptide 1 adjuvated with CFA/IFA or SFASE induces high titer antibodies cross-reactive with hVEGF165. (B) Immunization-induced antibodies inhibit the binding of bevacizumab to hVEGF165 in a competition ELISA. Antisera were used in 1:400 dilutions. (C) Antisera obtained 9 wk after the final immunization (in 1:100 dilution) coincubated with 1.2 ng/mL hVEGF165 inhibit the proliferation of Ba/F3–VEGFR2 cells. Antisera used in 1:200 dilutions gave similar results.