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. 2016 Oct 3;113(44):E6806–E6812. doi: 10.1073/pnas.1607152113

Fig. 5.

Fig. 5.

SAT1 contributes to p53-mediated ferroptosis upon ROS stress. (A) qRT-PCR analysis of SAT1 mRNA levels in stable U2OS control (Ctrl) CRISPR and SAT1 CRISPR cell lines treated with 10 μM Nutlin for the indicated times. (B) U2OS control CRISPR and SAT1 CRISPR stable cell lines were treated with 10 μM Nutlin for the indicated times, and total protein lysates were subjected to Western blot analysis for the expression of p53, PUMA, p21, and Actin. (C) Images of U2OS control CRISPR and SAT1 CRISPR cells treated with 10 μM Nutlin and 350 μM TBH for 24 h. (D) Quantification of cell death in C from three technical triplicates. Data are shown as mean ± SD. ***P < 0.001. (E) MEFs from the indicated genotypes were treated with 10 μM Nutlin, and Sat1 transcript levels were measured by qRT-PCR.