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. 2016 Oct 14;113(44):E6813–E6822. doi: 10.1073/pnas.1608210113

Fig. S10.

Fig. S10.

FHL2 nuclear localization at the active RNA polymerase II site in HFF cells on soft substrate. The images were obtained with a Nikon N-SIM superresolution microscope. (A) FHL2-GFP localization at the nucleus in an HFF cell on 8.78-kPa ACA gel coated with fibronectin. (A′) Active RNA polymerase II staining at the nucleus in an HFF cell on 8.78-kPa ACA gel coated with fibronectin. (A′′) Merged image of A and A′. (B) FHL2-GFP localization at the nucleus in an HFF cell on 75.3-kPa ACA gel coated with fibronectin. (B′) RNA polymerase II staining at the nucleus in an HFF cell on 75.3-kPa ACA gel coated with fibronectin. (B′′) Merged image of A and A′. (C) FHL2-GFP localization at the nucleus in an HFF cell on glass coated with fibronectin. (C′) CENP-A staining at the nucleus in an HFF cell on glass coated with fibronectin. (C′′) Merged image of C and C′. (D) Graph showing the Pearson correlation coefficient between FHL2-GFP and active RNA polymerase II staining at the nucleus in HFF cells on 8.78-kPa and 75.3-kPa ACA gels coated with fibronectin. (E) Graph showing the Pearson correlation coefficient between FHL2-GFP and CENP-A staining at the nucleus in HFF cells on fibronectin-coated glass. n > 20. Error bars represent SEM. ***P < 0.0001.