Fig. 3.

Representative current versus time traces for the various stages of the pore assembly. (A) When neither tagged nucleotide nor polymerase is present, only stable open-channel current is observable. (B) Attachment of polymerase does not change the mean open-channel current. The current root-mean-square fluctuation (RMSF) increase in B may be an indication of the polymerase coupled to the pore. (C) When no polymerase is attached to the pore and tagged nucleotide is introduced, transient events are observed. (D) When polymerase–template is attached to the pore and the complementary base dG6P-dT30 is added, there are prolonged capture events as well as transient events as observed in C.