Fig. 4.

Tagged-nucleotide discrimination on a semiconductor chip array. All measurements were taken on a pore–polymerase–template complex under noncatalytic conditions where the first base on the template is complementary to the added tagged nucleotide. (A) Current versus dwell time (duration of each current blockade) plots for captures of all tagged nucleotides. Capture events cluster into distinct current and dwell time regions for each tagged nucleotide. (B) Representative single-pore traces of tagged-nucleotide capture shown in A. Current blockade levels for each are marked in red. The blockades demonstrate unique, single-molecule events corresponding to the four distinct tag captures.