Fig. S4.

Calcium Green-1, AM fluorescence in WT and H82 cells (A), the effect of 1,2-bis(o-aminophenoxy)ethane-N,N,N,N′-tetraacetic acid (BAPTA) or N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (W-7) on the fluorescence of Calcium Green-1, AM (B). (A) Cells were grown in high-CO₂ (HC) or low-CO₂ (LC) conditions for 2 h or 12 h in light. Cells in LC-light (12 h) were transferred to LC-dark conditions for 2 h. (Scale bars: 5 µm.) (B) Cells were grown in LC-light conditions for 2 h. The chemical being tested was added to fresh medium before shifting from HC to LC conditions. CG-1 and CHL represent fluorescence derived from Calcium Green-1, AM and chlorophyll, respectively. Each image is placed with the flagella facing upward on the panel. DIC, differential interference contrast image. (Scale bars: 5 µm.)