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. 2016 Oct 11;113(44):12514–12519. doi: 10.1073/pnas.1613884113

Fig. S1.

Fig. S1.

Characterization of heterozygous R26-Cas9iGFP transgenic mice. (A) FACS plots of T cells (CD3+), myeloid cells (CD11b+), and B cells (CD19+) isolated from the mLNs of Cas9 transgenic or wild-type animals. (B) FACS plots of B cells (CD19+) isolated from the peritoneal cavity and spleen of Cas9 transgenic or wild-type animals. Gates were set on B1 and B2 cells in the spleen and B2, B1a, and B1b in the peritoneal cavity based on CD19, B220, CD43, CD5, and IgM markers as indicated. (C) FACS plots of B220+CD19+FashiCD38low GC B cells isolated from the spleen and mLNs. Data are representative of four independent experiments. Cells from wild-type C57BL/6 animals (red) and R26-Cas9iGFP/+ animals (blue) are shown in the same FACS plots.