Fig. S4.

Knockout efficiencies in Cas9-expressing B cells. (A) Selection of sgRNAs designed with CrispRGold targeting 12 B-cell surface makers. Targeting sequences are shown 5′–3′. (B) FACS plots showing the knockout efficiencies in Cas9-expressing B cells 4 d after transduction with sgRNAs against the indicated surface markers. Note that some surface markers are only induced upon activation (CD83 and Tnfrsf13b). The sgRNAs used are indicated in the title of each FACS plot. The data are representative of three independent experiments.