Fig. S6.

Robust detection of transcription factors important for B-cell activation and differentiation. (A) Scheme of B-cell activation and differentiation. Splenic B cells were activated with anti-CD40⁺IL4 and subsequently differentiated into plasmablasts using IL-21 and CD40-ligand expressing feeder cells. (B) sgRNAs used to target Prdm1, Xbp1, Pou2af1, Irf4, and Myc. (C) Analysis of the percentage of GFP⁺ Cas9-expressing B cells and GFP⁻ wild-type B cells. These cells were mixed, activated, and transduced (individually) with three sgRNAs per gene targeting Prdm1, Xbp1, Pou2af1, Irf4, and Myc. The FACS plots show the GFP⁺ Cas9-expressing B cells 4 and 8 d after transduction. (D) Analysis of the induction of CD138 in GFP⁺ Cas9-expressing and GFP⁻ wild-type B cells 6 d after transduction. The data are representative of three independent experiments.