Fig. S9.

Validation of candidates important for B-cell survival/proliferation and plasma cell differentiation. (A) FACS data showing the percentage of GFP⁺ Cas9-expressing and GFP⁻ wild-type B cells 2, 4, 6, and 8 d after transduction with the indicated sgRNAs. As before, Cas9-expressing B cells were mixed with wild-type B cells, activated, and transduced with retroviral particles expressing sgRNA targeting Rosa26 (as control), Myc, Egr2, Dis3, Ost4, Preb, and Pomp. (B) Analysis of the induction of CD138 in GFP⁺ Cas9-expressing and GFP⁻ wild-type B cells. Cas9-expressing B cells were mixed with wild-type B cells, activated, and transduced with retroviral particles expressing sgRNAs targeting Rosa26 (as control), Prdm1, Ost4, Arf4, Creld2, Zfp36, and Edem1.